mouse igg1 anti-nav1.6  (NeuroMab)

Journal: bioRxiv

Article Title: Differential encoding of mammalian proprioception by voltage-gated sodium channels

doi: 10.1101/2024.08.27.609982

Figure Lengend Snippet:

Article Snippet: For immunolabeling of NaVs the following primary antibodies were used: guinea pig anti-VGLUT1 (1:8000, Zuckerman Institute 1705), rabbit anti-NaV1.1 (2ug/ml, Neuromab A11954), mouse IgG1 anti-NaV1.6, mouse (6ug/ml, Neuromab K87A/10.2), rabbit anti-NaV1.6 (1:750, Alomone, ASC009), IgG2a anti-Ankyrin G (6ug/ml, N106/36.1), and chicken anti-neurofilament heavy (1:3000, Abcam ab4680).

Techniques:

Journal: Neural Plasticity

Article Title: Changes in the Fluorescence Tracking of NaV1.6 Protein Expression in a BTBR T+Itpr3tf/J Autistic Mouse Model

doi: 10.1155/2019/4893103

Figure Lengend Snippet: Altered NaV1.6 expression and distribution in the PFC of the BTBR mouse model. (a, b) The red channel represents confocal images of NaV1.6 (antisodium channel), the blue channel represents ankyrin-G (Ank-G), and the green channel represents NeuN immunofluorescence in the PFC of C57BL/6J and BTBR mice. (c) The mask ROI was used to detect NaV1.6 expression within the NeuN soma. (d) The quantification of NaV1.6 immunofluorescence expression within the soma of C57BL/6J and BTBR mice. n = 3 mice per group. (e) Representative confocal images of NaV1.6 (red) at the AIS in C57BL/6J and BTBR mice. (f) AIS tracking analysis of NaV1.6 in C57BL/6J and BTBR mice. n = 3 mice per group. (g) NaV1.6 protein levels in PFC lysates from C57BL/6J and BTBR mice and the Western blot bands. The protein expression was normalized with β -actin. n = 4-5 mice per group. Data represent mean ± SEM; statistical differences were assessed using Student's t -test ( ∗ p < 0.05). Scale bars represent 20 μ m in (b) and 10 μ m in the white box (zoom images in (b)) and (e).

Article Snippet: The primary antibodies used were mouse anti-FGF14 (1 : 300, NeuroMab, catalog number 75-096), mouse anti-ankyrin-G (1 : 1000, NeuroMab, catalog number 75-146), guinea pig anti-NeuN (1 : 750, Synaptic System, catalog number 266 004), mouse anti-NaV1.1 (1 : 500, NeuroMab, catalog number 75-023), mouse anti-NaV1.2 (1 : 300, NeuroMab, catalog number 75-024), mouse anti-NaV1.6 (1 : 300, NeuroMab, catalog number 75-026), mouse anti-Caspr (1 : 500, NeuroMab, catalog number 75-001), and mouse anti-PanNaV1 (1 : 300, NeuroMab, catalog number 75-405).

Techniques: Expressing, Immunofluorescence, Western Blot

Journal: Neural Plasticity

Article Title: Changes in the Fluorescence Tracking of NaV1.6 Protein Expression in a BTBR T+Itpr3tf/J Autistic Mouse Model

doi: 10.1155/2019/4893103

Figure Lengend Snippet: PanNaV, NaV1.1, and NaV1.2 expression in the PFC of the BTBR mouse model. (a, b) High-resolution confocal images where the red channel represents confocal images of PanNaV, the blue channel represents FGF14, and the green channel represents NeuN immunofluorescence in the PFC of C57BL/6J and BTBR mice. (c) Western blot analysis for PanNaV, NaV1.1, and NaV1.2 was performed on PFC homogenate and quantified. (d) The upregulation of PanNaV, NaV1.1, and NaV1.2. The protein expression was normalized with β -actin. Data represent mean ± SEM; statistical differences were assessed using Student's t -test ( ∗ p < 0.05, n = 4-5 mice per group). Scale bars represent 20 μ m in (b) and 10 μ m in zoom images in (b).

Article Snippet: The primary antibodies used were mouse anti-FGF14 (1 : 300, NeuroMab, catalog number 75-096), mouse anti-ankyrin-G (1 : 1000, NeuroMab, catalog number 75-146), guinea pig anti-NeuN (1 : 750, Synaptic System, catalog number 266 004), mouse anti-NaV1.1 (1 : 500, NeuroMab, catalog number 75-023), mouse anti-NaV1.2 (1 : 300, NeuroMab, catalog number 75-024), mouse anti-NaV1.6 (1 : 300, NeuroMab, catalog number 75-026), mouse anti-Caspr (1 : 500, NeuroMab, catalog number 75-001), and mouse anti-PanNaV1 (1 : 300, NeuroMab, catalog number 75-405).

Techniques: Expressing, Immunofluorescence, Western Blot

Journal: Neuron

Article Title: Macrophages Expressing GALC Improve Peripheral Krabbe Disease by a Mechanism Independent of Cross-Correction

doi: 10.1016/j.neuron.2020.03.031

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse anti-Nav1.6 clone K87A/10 , UC Davis / NeuroMab , RRID:MMRRC_06 5966-UCD.

Techniques: Immunocytochemistry, Virus, Recombinant, Western Blot, Mass Spectrometry, Plasmid Preparation, CCK-8 Assay, Cholesterol Assay, Reverse Transcription, Gene Expression, Derivative Assay, Cloning, Software